Application_advice:WB 1:1000-3000, IF/ICC 1:100-1:500
Immunogen:A synthesized peptide derived from human Smad3 (Phospho-Ser423).
Specificity:Smad3 (Phospho-Ser423) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser423.
Properties:If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification:The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage_Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.