Size

1 mg

Catalog no.

70R-FG001

Price

358 EUR

Isotypes:

IgG

Raised in:

Goat

Shipping conditions:

Blue Ice

French translation:

anticorps

Concentration:

1.0 mg/ml

Area of research:

Miscellaneous

Category:

Primary Antibody

Storage:

Store at 4 deg C.

Conjugation:

Anti-FITC Antibody

Tested for:

ELISA; ICC; IHC; WB

Antibody Subtype:

Polyclonal Antibodies, Purified

Cross Reactivity:

Should be determined by the end user

Form & Buffer:

Provided as a liquid in PBS, pH 7.2, with 0.1% NaN3.

Method of Purification:

FITC antibody was purified by affinity chromatography.

Type of Immunogen:

FITC antibodies were raised in goat using fluorescein ..

Usage Recommendations:

ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.