1 mg
70R-FG001
358 EUR
Isotypes:
IgG
Raised in:
Goat
Shipping conditions:
Blue Ice
French translation:
anticorps
Concentration:
1.0 mg/ml
Area of research:
Miscellaneous
Category:
Primary Antibody
Storage:
Store at 4 deg C.
Conjugation:
Anti-FITC Antibody
Tested for:
ELISA; ICC; IHC; WB
Antibody Subtype:
Polyclonal Antibodies, Purified
Cross Reactivity:
Should be determined by the end user
Form & Buffer:
Provided as a liquid in PBS, pH 7.2, with 0.1% NaN3.
Method of Purification:
FITC antibody was purified by affinity chromatography.
Type of Immunogen:
FITC antibodies were raised in goat using fluorescein ..
Usage Recommendations:
ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
Properties:
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.