1x96 well plate
Minutes of TMB incubation:15-20
Gen protein identity:P20693
ELISA's sensitivity:< 15pg/ml
Gene tested:murine CD23
Latin name:Mus musculus
Anti-coagulation:heparin or EDTA
ELISA kit detection limits:156pg/ml to 10000pg/ml
Target of this ELISA:Natural and recombinant mouse CD23
Uniprot:Low affinity immunoglobulin epsilon Fc receptor
Gene name:Fc fragment of IgE, low affinity II, receptor for (CD23)
Cross reaction:the ELISA does not cross react with any other proteins except from closely related species
Detector and capure antibodies:A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Keep at:Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Properties:E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA's application:For quantitative measurement of mouse CD23 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curve:Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.021 + 0.045 + 0.137 + 0.279 + 0.537 + 1.009 + 1.503 + 2.141
ELISA's precision parameters:intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);485;1882;8430;597;2347;9105 ELISA standard deviations 24.25;105.40;522.66;42.39;185.41;783.03 CV(%);5.0;5.6;6.2;7.1;7.9;8.6
Alternative:BLAST 2;BLAST-2;C-type lectin domain family 4 member J;CD23;CD23 antigen;CD23A;CLEC4J;Fc fragment of IgE low affinity II receptor for (CD23);Fc-epsilon-RII;FCE2;Fcer2;FCER2_HUMAN;IGEBF;Immunoglobulin E-binding factor;Low affinity immunoglobulin epsilon Fc receptor;Low affinity immunoglobulin epsilon Fc receptor soluble form;Lymphocyte IgE receptor
How the elisa works:Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse CD23 standard solution and each sample be measured in duplicate.
The ELISA contains:Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse CD23 antibodies;1 ST0000-10;Lyophilized recombinant mouse CD23 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse CD23 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Test:ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
ELISA kit description:GENTAUR's mouse CD23 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and your samples are measured with a biotin conjugated polyclonal from goat specific for CD23 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CD23 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
More gene info:CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibodies isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.