Tested applications:WB, IHC-P
Concentration:1ug per 1ul
Additional conjugation:HRP Conjugated
Crossreactivity:Human, Mouse, Rat
Target Antigen:hydrogen exchanger 3
Category:Conjugated Primary Antibodies
Host Organism:Rabbit (Oryctolagus cuniculus)
Recommended dilutions:WB(1:100-1000), IHC-P(1:100-500)
Purification method:This antibody was purified via Protein A.
Source:KLH conjugated synthetic peptide derived from human NHE3
Long name:hydrogen exchanger 3 Polyclonal Antibody, HRP Conjugated
Specificity:This is a highly specific antibody against hydrogen exchanger 3.
Crossreactive species details:Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Storage conditions:Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Synonyms:Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.
Properties:If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Antigen background:NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.