Net weight (g):0.1
Total weight (kg):1.4
Country of production:Canada
Verified applications:WB, AM
Stock availabilit:In Stock
Tested reactivity:To be tested
Tested applications:To be tested
PubMed number:Refer to PubMed
Bibliography:Refer to PubMed
Shipping recommendations:Blue Ice or 4°C
Representative figure link:No Data Available
Antibody's specificity:Detects 88.987 kDa.
Antibody's category:Polyclonal Antibodies
Latin name:Oryctolagus cuniculus
Antibody's target:Human MARK1 (pThr215)
Antibody in cell:Cytoplasm, Cytoskeleton
Antibody's purified from:Peptide Affinity Purified
Group:Polyclonals and antibodies
Recommended buffer for storage:PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's full description:Rabbit Anti-Human MARK1 (pThr215) Polyclonal
Antibody's concentration:See included datasheet or contact our support service
Scientific context:See included datasheet or contact our support service.
Antibody's recommended dilutions for use:WB (1:250); optimal dilutions for assays should be determined by the user.
Tissue specificity:Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Test:StressMark antibodies supplies antibodies that are for research of human proteins.
Antibody's datasheet:Contact our support service to receive datasheet or other technical documentation.
Immunogen:A phospho-specific peptide corresponding to residues surrounding Thr215 of human MARK1 (AA212-218)
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Certificate of analysis:A 1:250 dilution of SPC-1018 was sufficient for detection of MARK1 (pThr215) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody's other name:MAP/microtubule affinity regulating kinase 1 antibody, MAP/microtubule affinity-regulating kinase 1 antibody, MARK 1 antibody, MARK antibody, Mark1 antibody, MARK1_HUMAN antibody, Serine/threonine protein kinase MARK1 antibody, Serine/threonine-protein kinase MARK1 antibody
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.