HSP, Heat shock cognate protein 70, Affinity purified

Molecular weight (expected | аpparent):

70 kDa

Raised in:

Rabbit

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

P08107 , P11142

Connected products:

Secondary antibodies

Purification:

Affinity purified serum

Available ordering format:

Lyophilized in PBS pH 7.4

Verified applications:

Western blot (WB), immunoprecipitation (IP)

How to reconstitute:

For reconstitution add 50 µl of sterile water

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information:

this antibody is not suitable for work with samples from higher plants

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Possible reactivity:

vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster

Recommended dilutions for use:

1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)

Notes:

for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371

Immunogen:

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142

Test:

A high affinity purification column was use to purify HSP, Heat shock cognate protein 70, by agisera by chromatographic size exclusion.

Verified reactivity:

fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba

References:

French et al. (2015). High survivorship after catch-and-release fishing suggests physiological resilience in the endothermic shortfin mako shark (Isurus oxyrinchus). Conservation Physiology, Vol 3, Issue 1, 10.1093/conphys/cov044

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8