PsbA, D1 protein of PSII, C-terminal (affinity purified antibody)

Raised in:

Rabbit

French translation:

anticorps

TAIR number:

ATCG00020

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

38 | 28-30 kDa

Verified applications:

western blot (WB)

Purification:

Affinity purified serum

Available ordering format:

Lyophilized in PBS pH 7.4

Recommended dilutions for use:

1: 15 000 with standard ECL (WB)

How to reconstitute:

For reconstitution add 50 µl of sterile water

Protein number:

P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660

References:

to be added when available, antibody released in March 2015

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Properties:

If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Test:

A high affinity purification column was use to purify PsbA, D1 protein of PSII, C-terminal ( antibody) by agisera by chromatographic size exclusion.

Possible reactivity:

Medicago sativa, Pisum sativum, Triticum aestivum and other di and monocots,conifers, brown and red algae, cyanobacteria; cellular [compartment marker] of thylakoid membrane

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Immunogen:

KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Scientific context:

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Connected products:

AS01 016S | PsbA protein standard for a quantitative western blotAS05 084PRE | PsbA | D1 protein of PSII, C-terminal , pre-immune serumAS11 1786 | anti-PsbA N-terminal, rabbit antibodyAS10 704 | anti-PsbA | D1 protein of PSII, DE-loop, rabbit antibodyAS13 2669 | anti-PsbA | D1 protein of PSII, phosphorylated, rabbit antibodyAS05 084A-HRP | anti-PsbA | D1 protein of PSII, rabbit antibody directly conjugated to HRPrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies

Verified reactivity:

Arabidopsis thaliana, Artemisia annua, Arundo sp., Colobanthus quitensis Kunt Bartl, Craterostigma sp. Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana,Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Zea mays, Physcomitrella patens, Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942, Anabaena 7120, Paulinella chromatophora (amoeba), Prochlorococcus sp. (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp., Coscinodiscus wailesii, Ditylum brightwellii

Supplementary information:

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.

Notes:

Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.