LOX, Lipoxygenase (Affinity purified)

Notes:

None

Raised in:

Rabbit

Protein number:

P08170

Clonality:

Polyclonal

Clone:

Polyclonal

Recommended dilutions for use:

1:5000 (WB)

TAIR number:

Refer to NCBI

Verified applications:

western blot (WB)

Available ordering format:

Lyophilized PBS pH 7.4

Purification:

Affinity purified serum

Verified reactivity:

Arabidopsis thaliana, Glycine max

Molecular weight (expected | аpparent):

54 (subunit), 108 (native enzyme)

Supplementary information:

Contact Gentaur support center at [email protected]

Possible reactivity:

Lathyrus undulatus, Malus x domestica, Solanum tuberosum

References:

to be added when available, antibody released in December 2014

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

How to reconstitute:

For reconstitution of lyophilized unit please add 50 µl of sterile water.

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Immunogen:

native lipoxygenase, type I-B, purified from Glycine max (Sigma, product number L7395) P08170

Test:

A high affinity purification column was use to purify LOX, Lipoxygenase ( ) by agisera by chromatographic size exclusion.

Connected products:

AS06 128 | anti-LOX | lipoxygenase, rabbit antibodies (not purified serum)AS07 258 | anti-LOX-C | chloroplastic lipoxygenaseAS08 340 | anti-LOX2 | lipoxygenase 2 proteinPlant protein extraction buffer Secondary antibodies

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals).Synonymes: linoleate, oxygen oxidoreductase.