RbcS, Rubisco small subunit (SSU) (affinity purified)

Notes:

None

Raised in:

Rabbit

Protein number:

P10796

TAIR number:

AT5G38430

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

20 | 15 kDa

No reactivity:

cyanobacteria

Purification:

Affinity purified

Recommended dilutions for use:

1: 1000 with ECL (WB)

Available ordering format:

Lyophilized in PBS pH 7.4

Supplementary information:

to be added when available

Verified applications:

ELISA (ELISA), Western Blot (WB)

How to reconstitute:

For reconstitution add 50 µl of sterile water.

References:

to be added when available, antibody released in March 2016.

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Verified reactivity:

Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Malus domestica, Nicotiana tabacum

Test:

A high affinity purification column was use to purify RbcS, Rubisco subunit (SSU) ( ) by agisera by chromatographic size exclusion.

Immunogen:

KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1B of Arabidopsis thaliana At5g38430

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.

Possible reactivity:

Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea mays, liverwort Marchantia paleacea, green algae

Connected products:

AS07 259 | RbcS | Rubisco small subunit (SSU)AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated) AS15 2955 | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodyAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017 | anti-RbcL | Rubisco large subunit, form I, chicken antibodyAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS09 409 | Rubisco quantitation kit AS15 2994 | Rubisco ELISA quantitation kit AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody matching secondary antibody