UBQ11, Ubiquitin, affinity purified antibody

Notes:

None

No reactivity:

algae

Raised in:

Rabbit

Molecular weight (expected | аpparent):

8.5 kDa

French translation:

anticorps

TAIR number:

AT4G05050

Clonality:

Polyclonal

Clone:

Polyclonal

Available ordering format:

Lyophilized

Protein number:

Refer to NCBI

Verified applications:

Western blot (WB)

Purification:

Affinity purified serum

Recommended dilutions for use:

1: 10 000 with standard ECL (WB)

How to reconstitute:

For reconstitution add 50 µl of sterile water

Verified reactivity:

Arabidopsis thaliana, Nicotiana benthamina, Oryza sativa

References:

to be added when available, antibody released in November 2013.

Supplementary information:

antibody can be used to check qubiquitination status in the whole plant extracts.

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Immunogen:

recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR: At4g05050

Possible reactivity:

dicots including: Nicotiana tabacum, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, Zea mays

Test:

A high affinity purification column was use to purify UBQ11, Ubiquitin, antibody by agisera by chromatographic size exclusion.

Connected products:

AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum formatAS10 307S | UBQ11 | Ubiquitin protein | positive control

Properties:

If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Scientific context:

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.