Molecular weight (expected | аpparent):38.9 kDa
TAIR number:Refer to NCBI
Protein number:Refer to NCBI
Verified reactivity:Carica papaya
Possible reactivity:Carica papaya
Purification:Affinity purified IgG
References:to be added when available
Available ordering format:Lyophilized in phosphate buffer saline
Recommended dilutions for use:1: 1000 - 1: 100 000 for techniques listed above
Immunogen:native papain isolated and purified from Carica papaya
How to reconstitute:For reconstitution please add 0.5 ml of sterile destilled water.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Supplementary information:Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Test:A high affinity purification column was use to purify PPI, papain, by agisera by chromatographic size exclusion.
Connected products:AS09 545 | anti-PPI | papainAS09 546 | anti-PPI | papain (biotinylated)Plant protein extraction bufferSecondary antibodies
Notes:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Scientific context:Papain (EC=18.104.22.168) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.