HRP, horseradish peroxidase, Affinity purified

Raised in:

Rabbit

Molecular weight (expected | аpparent):

40 kDa

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Conjugation:

HRP Conjugated

Possible reactivity:

Armoracia rusticana

Verified reactivity:

Armoracia rusticana

Purification:

Affinity purified IgG

References:

to be added when available

Available ordering format:

Lyophilized in phosphate buffer saline

Recommended dilutions for use:

1: 1000 - 1: 10 000 for techniques listed above

Immunogen:

native peroxidase isolated and purified from horseradish

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Connected products:

AS09 548| anti-HRP horseradishPlant protein extraction bufferSecondary antibodies

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify horseradish by agisera by chromatographic size exclusion.

Conjugate:

Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Properties:

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Scientific context:

Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.