PLD, phospholipase D, Affinity purified

Molecular weight (expected | аpparent):

92 kDa

Raised in:

Rabbit

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Verified reactivity:

Brassica oleracea

Purification:

Affinity purified IgG

References:

to be added when available

Available ordering format:

Lyophilized in phosphate buffer saline

Recommended dilutions for use:

1: 100 - 1: 1000 for techniques listed above

Immunogen:

phospholipase D isolated and purified from Brassica oleracea

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Possible reactivity:

dicots including: Arabidopsis thaliana, Cucumis melo, Ricinus communis, trees: Populus balsamifera

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify PLD, phospholipase D, by agisera by chromatographic size exclusion.

Connected products:

AS09 556 | anti-PLD phospholipase DAS09 557 | anti-PLD phospholipase D (biotinylated)Plant protein extraction bufferSecondary antibodies

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.