Size

1 mg

Catalog no.

AS10 1274

Price

344 EUR

Group:

Sera

Available ordering format:

Liquid

Raised in:

Rabbit

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

References:

Refer to PubMed

Storage condition:

Store at 2-8°C.

Verified reactivity:

to be determined

Possible reactivity:

to be determined

Immunogen:

to be determined

No reactivity:

to be determined

Conjugation:

not conjugated 

Purification:

Affinity purified IgG

Latin name:

Oryctolagus cuniculus

Verified applications:

Contact Gentaur support center

Connected products:

other unconjugated antibodies to goat IgG

Supplementary information:

Contact Gentaur support center at [email protected]

How to reconstitute:

See included datasheet or contact our support service

Recommended dilutions for use:

Needs to be determined by end user for all immunoassay applications.

Test:

A high affinity purification column was use to purify Rabbit, Unconjugated, min. cross-reactivity serum by agisera by chromatographic size exclusion.

Scientific context:

Rabbit anti-goat IgG Fc, affinity purified, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are adsorbed against human serum and are affinity purified using solid phase goat IgG.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About:

Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Notes:

This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.