Molecular weight (expected | аpparent):15 | 17 kDa
Verified reactivity:Arabidopsis thaliana
Purification:Affinity purified serum
Protein number:P59169 , P59226 , Q9FXI7
Available ordering format:Lyophilized in PBS pH 7.4
Verified applications:ChIp-qPCR (ChIp-qPCR), western blot (WB)
How to reconstitute:For reconstitution add 50 µl of sterile water.
References:To be added when available, antibody released in April 2016.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Recommended dilutions for use:1 : 5000 with standard ECL (WB), 2.5 µg/100 µg of chromatin (ChIp-qPCR)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Notes:cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
Scientific context:Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Test:A high affinity purification column was use to purify H3, Histone H3 ( ) by agisera by chromatographic size exclusion.
Supplementary information:Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific protol for ChIP can be found here: Saleh et al. (2008).
Immunogen:KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)
Storage condition:Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Connected products:AS11 1804 | anti-RNA polymerase II subunit B1, rabbit antibodyAS16 3197 | anti-H3 | Histone 3 core, monoclonal antibodyAS15 2855 | anti-H3 | histone H3, chicken antibodyAS10 710 | anti-H3 | histone H3, rabbit antibody (serum)AS10 710PRE | H3 | histone H3, pre-immune serumCollection of antibodies to epigenetics/DNA methylationPlant and algal protein extraction buffer Secondary antibodies
Possible reactivity:Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.