NBR1, Autophagy substrate NBR1 (affinity purified)

Notes:

None

Raised in:

Rabbit

Protein number:

Q9SB64

TAIR number:

AT4G24690

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

75 | 100 kDa

Verified applications:

western blot (WB)

Possible reactivity:

Brassicaceae family

Verified reactivity:

Arabidopsis thaliana

Purification:

Affinity purified serum

Available ordering format:

Lyophilized in PBS pH 7.4

Recommended dilutions for use:

1 : 500 - 1: 5000 with standard ECL (WB)

How to reconstitute:

For reconstitution add 50 µl of sterile water.

References:

to be added when available, antibody release in February 2015

No reactivity:

no confirmed exceptions from predicted reactivity are currently known

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Immunogen:

UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Test:

A high affinity purification column was use to purify NBR1, Autophagy substrate NBR1 ( ) by agisera by chromatographic size exclusion.

Supplementary information:

specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

Connected products:

AS14 2805 | anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies in serum format AS14 2769 | anti-ATG8, rabbit antibodiesAS14 2811 | anti-ATG8A | Autophagy-related protein 8a, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.