Molecular weight (expected | аpparent):75 | 100 kDa
Verified applications:western blot (WB)
Possible reactivity:Brassicaceae family
Verified reactivity:Arabidopsis thaliana
Purification:Affinity purified serum
Available ordering format:Lyophilized in PBS pH 7.4
Recommended dilutions for use:1 : 500 - 1: 5000 with standard ECL (WB)
How to reconstitute:For reconstitution add 50 µl of sterile water.
References:to be added when available, antibody release in February 2015
No reactivity:no confirmed exceptions from predicted reactivity are currently known
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Immunogen:UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
Test:A high affinity purification column was use to purify NBR1, Autophagy substrate NBR1 ( ) by agisera by chromatographic size exclusion.
Supplementary information:specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.
Connected products:AS14 2805 | anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies in serum format AS14 2769 | anti-ATG8, rabbit antibodiesAS14 2811 | anti-ATG8A | Autophagy-related protein 8a, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Scientific context:NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.