Concentration:1ug per 1ul
Cross reactivity:Human, Mouse, Rat
Clone number:Polyclonal antibody
Modification site(s):Unmodified antibody
Target Antigen:hydrogen exchanger 3
Tested Applications:WB, IHC-P, IF(IHC-P)
Also known as:hydrogen exchanger 3 PAb
Purification method:This antibody was purified via Protein A.
Long name:hydrogen exchanger 3 Primary Polyclonal Antibody
Recommended dilutions:WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Specificity:This is a highly specific antibody against hydrogen exchanger 3.
Cross reactive species details:Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source:This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NHE3
Storage conditions:Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Properties:If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Synonym names:Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.
Background of the target antigen:NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.