Goat Anti-Mouse IgG(H+L) CY3-conjugated

Additional isotype:

IgG

Conjugate:

CY3

Reactivity:

Mouse

Format:

Liquid

Concentration:

1mg/ml

Shipping:

Ice bag

Clonality:

Polyclonal

Fragment:

Fab fragment

Application:

WB,IF/ICC,FCM

Application_advice:

1:100 – 1:500

Type:

secondary antibody

Conjugation:

cy3 conjugation kit

Purification:

Affinity-chromatography.

Latin name:

Capra aegagrus hircus,Mus musculus

Antigen:

Goat Anti-Mouse IgG(H+L) CY3-conjugated

Immunogen:

The antiserum was developed in goat using the Mouse IgG as the immunogen.

Storage_Buffer:

0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6._x000D_ Store at -20℃，avoid repeated thawing and freezing.

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties:

Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Goat Anti-Mouse IgG(H+L) -conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Affinity Biosciences antibody is well suited for fluorescent microscopy.

Test:

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.