Flag-Tag Antibody

Reactivity:

All

Conjugation:

Flag

Concentration:

1mg/ml

Format:

Liquid

Shipping:

Ice bag

Antigen:

Flag-Tag

French translation:

anticorps

Clonality:

Monoclonal

Fragment:

Fab fragment

Conjugate:

Unconjugated

Type:

primary antibody

Application:

WB,IHC,IF/ICC,IP,ELISA

Purification:

Affinity-chromatography.

Application_advice:

WB: 1:3000~1:10000, IF/ICC: 1:200,IP 1:200

Immunogen:

A synthetic peptide DYKDDDDK coupled to KLH.

Storage_Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Properties:

If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.