Goat Anti-Mouse IgG(H+L) FITC -conjugated

Additional isotype:

IgG

Conjugate:

FITC

Reactivity:

Mouse

Format:

Liquid

Concentration:

1mg/ml

Shipping:

Ice bag

Clonality:

Polyclonal

Fragment:

Fab fragment

Application:

WB,IF/ICC,FCM

Application_advice:

IF 1:100 – 1:500

Type:

secondary antibody

Conjugation:

Anti-FITC Antibody

Purification:

affinity purification.

Latin name:

Capra aegagrus hircus,Mus musculus

Antigen:

Goat Anti-Mouse IgG(H+L) FITC -conjugated

Immunogen:

The antiserum was developed in goat using the MOUSE IgG as the immunogen.

Storage_Buffer:

0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6._x000D_ Store at -20℃，avoid repeated thawing and freezing.

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test:

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Properties:

This Affinity Biosciences Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Affinity Biosciences FITC is highly sensitive to pH extremes.