Anti-rat IgG(H+L), HRP-linked Antibody

Application:

WB

Reactivity:

Rat

Additional isotype:

IgG

Conjugate:

HRP

Format:

Liquid

Concentration:

1mg/ml

Shipping:

Ice bag

French translation:

anticorps

Clonality:

Monoclonal

Fragment:

Fab fragment

Conjugation:

HRP Conjugated

Latin name:

Rattus norvegicus

Application_advice:

WB: 1:5000~1:10000

Type:

secondary antibody

Purification:

affinity purification.

Antigen:

Anti-rat IgG(H+L), HRP-linked

Immunogen:

The antiserum was developed in goat using the rat IgG as the immunogen.

Storage_Buffer:

IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties:

If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.