Biotin antibody

Isotypes:

IgG

Raised in:

Goat

Shipping conditions:

Blue Ice

French translation:

anticorps

Concentration:

1.0 mg/ml

Conjugation:

Biotinylated

Area of research:

Miscellaneous

Category:

Primary Antibody

Storage:

Store at 4 deg C.

Tested for:

ELISA; ICC; IHC; WB

Antibody Subtype:

Polyclonal Antibodies, Purified

Cross Reactivity:

Should be determined by the end user

Method of Purification:

Biotin antibody was purified by affinity chromatography.

Form & Buffer:

Affinity purified and Provided in PBS, pH 7.2, with 0.1% NaN3.

Usage Recommendations:

ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.