Biotin antibody

Raised in:

Goat

Shipping conditions:

Blue Ice

French translation:

anticorps

Concentration:

1.0 mg/ml

Conjugation:

Biotinylated

Area of research:

Miscellaneous

Tested for:

ELISA; IHC; WB

Category:

Primary Antibody

Antibody Subtype:

Polyclonal Antibodies, Purified

Cross Reactivity:

Should be determined by the end user

Form & Buffer:

0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Usage Recommendations:

ELISA: 1:20,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Type of Immunogen:

Biotin antibodies were raised in goat using biotin conjugated to KLH ..

Method of Purification:

Biotin antibody was purified by Ion exchange chromatography followed by dialysis.

Storage:

Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.