FLAG Antibody

Conjugation:

Flag

Raised in:

Chicken

Shipping conditions:

Blue Ice

French translation:

anticorps

Concentration:

100 ug/ml

Area of research:

Miscellaneous

Category:

Primary Antibody

Tested for:

Please refer to the datasheet

Antibody Subtype:

Polyclonal Antibodies, Purified

Method of Purification:

FLAG Antibody was purified by affinity chromatography.

Type of Immunogen:

a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin

Form & Buffer:

Provided in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% NaN3 added as a preservative.

Storage:

Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.