HA antibody FITC

Conjugate/Tag:

FITC

Concentration:

1 mg/ml

Raised in:

Chicken

Shipping conditions:

Blue Ice

French translation:

anticorps

Area of research:

Miscellaneous

Category:

Primary Antibody

Conjugation:

Anti-FITC Antibody

Tested for:

Please refer to the datasheet

Antibody Subtype:

Conjugated Polyclonal Antibodies

Method of Purification:

HA antibody (FITC) was purified by affinity chromatography.

Form & Buffer:

Provided in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% NaN3 added as an antimicrobial agent and 0.2 % BSA.

Type of Immunogen:

a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) conjugated to keyhole limpet hemocyanin

Storage:

Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.