Luciferase antibody FITC

Conjugate/Tag:

FITC

Raised in:

Rabbit

Specificity:

Firefly

Concentration:

50 ug/ml

Shipping conditions:

Blue Ice

French translation:

anticorps

Tested for:

ELISA; IHC

Category:

Primary Antibody

Conjugation:

Anti-FITC Antibody

Usage Recommendations:

ELISA: 1:10,000, IHC: 1:100

Antibody Subtype:

Conjugated Polyclonal Antibodies

Area of research:

Proteases, Inhibitors, & Enzymes

Cross Reactivity:

Specific for Firefly Luciferase (Photinus pyralis)

Form & Buffer:

Affinity purified and Provided in PBS, pH 7.4, with 0.2% BSA and 0.05% NaN3.

Type of Immunogen:

Luciferase antibody (FITC) was raised in rabbit using highly purified firefly luciferase ..

Storage:

Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.