Chicken FLAG Antibody

Clone:

NA

Cross Reactivity:

NA

Specificity:

NA

Conjugation:

Flag

Shipping Info:

Blue Ice

French translation:

anticorps

Concentration:

100 ug/ml

Research Area:

Miscellaneous

Applications:

User optimized

Product Type:

Primary Antibodies

Product Subtype:

Purified Polyclonal Antibodies

Immunogen:

a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin

Form & Buffer:

Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.

Storage:

Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.