Rabbit Luciferase antibody (FITC)

Clone:

NA

Specificity:

Firefly

Concentration:

50 ug/ml

Shipping Info:

Blue Ice

French translation:

anticorps

Applications:

ELISA, IHC

Conjugation:

Anti-FITC Antibody

Product Type:

Primary Antibodies

Latin name:

Oryctolagus cuniculus

Research Area:

Proteases, Inhibitors, & Enzymes

Product Subtype:

Conjugated Polyclonal Antibodies

Cross Reactivity:

Specific for Firefly Luciferase (Photinus pyralis)

Form & Buffer:

Affinity purified and supplied in PBS, pH 7.4, with 0.2% BSA and 0.05% NaN3.

Immunogen:

Luciferase antibody (FITC) was raised in rabbit using highly purified firefly luciferase as the immunogen.

Storage:

Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

About:

Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Properties:

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.