Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit

Assay Time:

3 hours

Shipping Conditions:

Ice packs

Sensitivity:

0.135 ng/mL

Detection Method:

Colorimetric

Detection Range:

0.312-20 ng/mL

Estimated Turnaround Time:

6-11 Business days

Species Reactivity:

Homo sapiens (Human)

Research Area:

CD & Adhesion molecule

Assay Type:

Double-antibody Sandwich

Storage Temperature:

Short term: 4°C; Long term: see manual.

Target Name:

Fc Fragment Of IgG Low Affinity IIIb Receptor

Application:

For research use only. Not for diagnostic procedures.

Precaution of Use:

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems:

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Sample Type:

Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids

Shelf life:

Use Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit before 8 months

Target's alterntive name:

CD16b, CD16-B, FCGR3-B, FCG3, FcgRIII, Low affinity immunoglobulin gamma Fc region receptor III-B

Specificity:

This assay has high sensitivity and excellent specificity for detection of Fc Fragment Of IgG Low Affinity IIIb Receptor

Test Principle:

No significant cross-reactivity or interference between Fc Fragment Of IgG Low Affinity IIIb Receptor and analogues was observed.

Cross Activity:

No significant cross-reactivity or interference between Fc Fragment Of IgG Low Affinity IIIb Receptor and analogues was observed.

Test:

A high affinity purification column was use to purify Fc Of IgG IIIb Receptor (FcgR3B) ELISA Kit by Biomatik by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Additional isotype:

Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG

Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fc Fragment Of IgG Low Affinity IIIb Receptor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fc Fragment Of IgG Low Affinity IIIb Receptor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Stability:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description:

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.