Rat High Affinity Choline Transporter (ChT) ELISA Kit

Assay Time:

3 hours

Sensitivity:

28 pg/mL

Shipping Conditions:

Ice packs

Detection Method:

Colorimetric

Detection Range:

78.12-5000 pg/mL

Latin name:

Rattus norvegicus

Estimated Turnaround Time:

6-11 Business days

Research Area:

Signal transduction

Species Reactivity:

Rattus norvegicus (Rat)

Assay Type:

Double-antibody Sandwich

Target Name:

High Affinity Choline Transporter

Storage Temperature:

Short term: 4°C; Long term: see manual.

Application:

For research use only. Not for diagnostic procedures.

Sample Type:

Tissue homogenates, Cell lysates and other biological fluids.

Precaution of Use:

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems:

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life:

Use Rat High Affinity Choline Transporter (ChT) ELISA Kit before 8 months

Specificity:

This assay has high sensitivity and excellent specificity for detection of High Affinity Choline Transporter

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cross Activity:

No significant cross-reactivity or interference between High Affinity Choline Transporter and analogues was observed.

Test Principle:

No significant cross-reactivity or interference between High Affinity Choline Transporter and analogues was observed.

Target's alterntive name:

SLC5A7, hCHT, CHT1, Solute Carrier Family 5 Member 7, Choline Transporter, Hemicholinium-3-sensitive choline transporter

Test:

A high affinity purification column was use to purify Choline Transporter (ChT) ELISA Kit by Biomatik by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Affinity Choline Transporter were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Affinity Choline Transporter were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.