Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit

Assay Time:

3 hours

Shipping Conditions:

Ice packs

Sensitivity:

0.064 ng/mL

Detection Method:

Colorimetric

Detection Range:

0.156-10 ng/mL

Latin name:

Rattus norvegicus

Estimated Turnaround Time:

6-11 Business days

Research Area:

Signal transduction

Species Reactivity:

Rattus norvegicus (Rat)

Assay Type:

Double-antibody Sandwich

Storage Temperature:

Short term: 4°C; Long term: see manual.

Target Name:

Rho Guanine Nucleotide Exchange Factor 7

Sample Type:

Tissue homogenates and other biological fluids.

Application:

For research use only. Not for diagnostic procedures.

Test:

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Precaution of Use:

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems:

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life:

Use Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit before 8 months

Specificity:

This assay has high sensitivity and excellent specificity for detection of Rho Guanine Nucleotide Exchange Factor 7

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cross Activity:

No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 7 and analogues was observed.

Test Principle:

No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 7 and analogues was observed.

Target's alterntive name:

PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Description:

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Stability:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.