Mouse Low Affinity Nerve Growth Factor Receptor (LNGFR) ELISA Kit

Tissue:

nerve

Assay Time:

3 hours

Shipping Conditions:

Ice packs

Sensitivity:

0.058 ng/mL

Latin name:

Mus musculus

Detection Method:

Colorimetric

Detection Range:

0.156-10 ng/mL

Estimated Turnaround Time:

6-11 Business days

Species Reactivity:

Mus musculus (Mouse)

Assay Type:

Double-antibody Sandwich

Storage Temperature:

Short term: 4°C; Long term: see manual.

Target Name:

Low Affinity Nerve Growth Factor Receptor

Application:

For research use only. Not for diagnostic procedures.

Research Area:

Signal transduction, Infection immunity, Neuro science

Sample Type:

Serum, Plasma, Tissue homogenates and other biological fluids

Precaution of Use:

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems:

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life:

Use Mouse Low Affinity Nerve Growth Factor Receptor (LNGFR) ELISA Kit before 8 months

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Specificity:

This assay has high sensitivity and excellent specificity for detection of Low Affinity Nerve Growth Factor Receptor

Cross Activity:

No significant cross-reactivity or interference between Low Affinity Nerve Growth Factor Receptor and analogues was observed.

Test Principle:

No significant cross-reactivity or interference between Low Affinity Nerve Growth Factor Receptor and analogues was observed.

Target's alterntive name:

CD271, NGFR, TNFRSF16, P75 (NTR), Tumor necrosis factor receptor superfamily member 16, P75 Neurotrophin Receptor, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR

Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Affinity Nerve Growth Factor Receptor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Affinity Nerve Growth Factor Receptor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Test:

A high affinity purification column was use to purify Mouse Nerve Growth Factor Receptor (LNGFR) ELISA Kit by Biomatik by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Description:

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.