96 Tests
EKE61038
759 EUR
Stability:
NA
Precision:
NA
Specificity:
NA
Target Name:
NA
Species Reactivity:
Human
Target's alterntive name:
FcγR3B
Assay Type:
Sandwich
Assay Time:
4.5 hours
Shipping Conditions:
Ice packs
Research Area:
Immunology
Sensitivity:
0.19 ng/mL
Detection Method:
Colorimetric
Detection Range:
0.31-20 ng/mL
Estimated Turnaround Time:
6-11 business days
Sample Type:
Serum, Plasma, Biological Fluids
Storage Temperature:
Short term: 4°C; Long term: see manual.
Application:
For research use only. Not for diagnostic procedures.
Quality Systems:
The kit is manufactured at ISO 9001 certified facilities.
Cross Activity:
No significant cross-reactivity or interference was observed.
Precaution of Use:
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life:
Use Human FcγR3B (Fc Fragment of IgG Low Affinity Ⅲb Receptor) ELISA Kit before 6 months
Test:
A high affinity purification column was use to purify FcγR3B (Fc of IgG Ⅲb Receptor) ELISA Kit by Biomatik by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Additional isotype:
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG
About:
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description:
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Test Principle:
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FcγR3B . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγR3B and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγR3B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FcγR3B. The concentration of Human FcγR3B in samples can be calculated by comparing the OD of the samples to the standard curve.