10x96T
EKU09352
7084 EUR
Verified applications:
ELISA
Assay duration:
3 hours
Use for:
8 months
Assay class:
Sandwich
Shipping requirements:
Blue ice
Sensitivity limit:
6,5 pg/mL
ELISA detection:
Colorimetric
Detection limits:
15.62-1000 pg/mL
Estimated production time:
7-11 business days
Verified reactivity:
Homo sapiens (Human)
Research main area:
CD & Adhesion molecule;
Gene number:
Please refer to GenBank
Protein number:
Please refer to Uniprot
Alternate protein number:
Please refer to SwissProt
Gene name:
Fc Fragment Of IgG Low Affinity IIb, Receptor
Protocol:
Please see ELISA's datasheet, otherwise contact us
Notes:
For research use only. Not for diagnostic procedures.
QC:
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Samples to be analyzed:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Alternate gene name:
CD32; FCG2; FCGR2; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-b
ELISA's cross-reactivity:
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Test:
A high affinity purification column was use to purify Fc Of IgG IIb, Receptor (FcgR2B) ELISA Kit, 96 by bioma by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Storage recommendation:
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Warnings:
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
ELISA's specificity:
This assay has high sensitivity and excellent specificity for detection of Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B). No significant cross-reactivity or interference between Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B) and analogues was observed.
Additional isotype:
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG
Precision of the test:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
About:
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
ELISA's stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Assay principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fc Fragment Of IgG Low Affinity IIb, Receptor (FcgR2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Description:
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.