Anion Exchange Protein 1 (AE1) Polyclonal Antibody (Rat), FITC

French translation:

anticorps

Latin name:

Rattus norvegicus

Conjugation:

Anti-FITC Antibody

Notes:

Research Use Only.

Group:

Polyclonals and antibodies

Description:

A Rabbit polyclonal antibody against Rat Anion Exchange Protein 1 (AE1). This antibody is labeled with FITC.

Additional_information:

Sequence of the immunogen: AE1 (Ile558~Phe675); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Storage_and_shipping:

Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.

Specifications:

Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Properties:

If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.