Affinity purified Mouse IgG1 Isotype Antibody BIOTIN-Conjugated

Additional isotype:

IgG1

Source:

Mouse

Conjugation:

BIOTIN

Clone:

MOPC-21

Group:

isotypes

Antibody's concentration:

0,5 mg/ml

French translation:

anticorps

Clonality:

Monoclonal

Additional conjugation:

Biotinylated

Latin name:

Mus musculus

Category:

Isotype Control

Purification:

Affinity purified

Reactivity:

Not specified / Inquire

Antibody's supplied with:

PBS pH 7.2, 0.09% NaN3, 0.2% BSA

Additional description:

1 of protein A or G purified by Elabscience will give more specificity than crude serum.

Storage:

Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

Description:

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Antibody's is used for:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

Test:

A high affinity purification column was use to purify Mouse IgG1 Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties:

If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.