Affinity purified Rat IgG1 Isotype Antibody FITC-Conjugated

Source:

Rat

Clone:

HRPN

Conjugation:

FITC

Additional isotype:

IgG1

Group:

isotypes

Antibody's concentration:

0,5 mg/ml

French translation:

anticorps

Clonality:

Monoclonal

Category:

Isotype Control

Purification:

Affinity purified

Latin name:

Rattus norvegicus

Additional conjugation:

Anti-FITC Antibody

Reactivity:

Not specified / Inquire

Antibody's supplied with:

PBS pH 7.2, 0.09% NaN3, 0.2% BSA

Additional description:

1 of protein A or G purified by Elabscience will give more specificity than crude serum.

Storage:

Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

Description:

Rat IgG1 was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Test:

A high affinity purification column was use to purify IgG1 Isotype Antibody FITC-Conjugated by Elabscience by chromatographic size exclusion.

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Antibody's is used for:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

Properties:

If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.