Affinity purified Rat IgG2b Isotype Antibody BIOTIN-Conjugated

Source:

Rat

Clone:

LTF-2

Additional isotype:

IgG2b

Conjugation:

BIOTIN

Group:

isotypes

Antibody's concentration:

0,5 mg/ml

French translation:

anticorps

Clonality:

Monoclonal

Additional conjugation:

Biotinylated

Category:

Isotype Control

Purification:

Affinity purified

Latin name:

Rattus norvegicus

Reactivity:

Not specified / Inquire

Antibody's supplied with:

PBS pH 7.2, 0.09% NaN3, 0.2% BSA

Additional description:

1 of protein A or G purified by Elabscience will give more specificity than crude serum.

Storage:

Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

Test:

A high affinity purification column was use to purify IgG2b Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.

Description:

Rat IgG2b was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Antibody's is used for:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

Properties:

If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.