Affinity purified Hamster IgG Isotype Antibody BIOTIN-Conjugated

Additional isotype:

IgG

Conjugation:

BIOTIN

Source:

Hamster

Group:

isotypes

French translation:

anticorps

Antibody's concentration:

0,5 mg/ml

Clonality:

Polyclonal

Clone:

Polyclonal

Additional conjugation:

Biotinylated

Category:

Isotype Control

Purification:

Affinity purified

Reactivity:

Not specified / Inquire

Antibody's supplied with:

PBS pH 7.2, 0.09% NaN3, 0.2% BSA

Additional description:

1 of protein A or G purified by Elabscience will give more specificity than crude serum.

Storage:

Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

Test:

A high affinity purification column was use to purify IgG Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.

Description:

The isotype of this antibody is Hamster IgG. This hamster IgG fraction was purified by affinity chromatography from Armenian hamster serum.

Antibody's is used for:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Properties:

If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.