200 mg
EHIS002-200
290 EUR
Additional isotype:
IgG
Conjugation:
FITC
Source:
Hamster
Group:
isotypes
French translation:
anticorps
Antibody's concentration:
0,5 mg/ml
Clonality:
Polyclonal
Clone:
Polyclonal
Category:
Isotype Control
Purification:
Affinity purified
Additional conjugation:
Anti-FITC Antibody
Reactivity:
Not specified / Inquire
Antibody's supplied with:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
Additional description:
1 of protein A or G purified by Elabscience will give more specificity than crude serum.
Storage:
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
Description:
The isotype of this antibody is Hamster IgG. This hamster IgG fraction was purified by affinity chromatography from Armenian hamster serum.
Test:
A high affinity purification column was use to purify IgG Isotype Antibody FITC-Conjugated by Elabscience by chromatographic size exclusion.
Antibody's is used for:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
About:
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Properties:
If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.