Recombinant Human CD16a / FCGR3A Protein (176 Val, His tag)

Fusion tag:

C-His

AP Mol Mass:

48 kDa

Mol Mass:

23.3 kDa

Conjugation:

histidine

Accession:

AAH17865.1

Group:

recombinants

Expressed Host:

HEK293 Cells

Sequence:

Met 1-Gln 208

Source:

Recombinants or rec. proteins

Purity:

＞97 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4

Endotoxin:

＜1.0 EU per µg as determined by the LAL method

Synonym:

CD16;CD16A;Fc gamma RIIIa;FCG3;FCGR3;FCGRIII;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Activity:

Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80nM.2.Measured by its binding ability in a functional ELISA. Immobilized human CD16a-His(176 Val) at 10ug/ml(100ul/well ) can bind human IgG1 with a linear range of 0.00128-0.8 μg/ml.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.