Recombinant Human CD32a / FCGR2A Protein (167 His, His & AVI tag), Biotinylated

AP Mol Mass:

33 kDa

Mol Mass:

23.6 kDa

Accession:

P12318-1

Conjugation:

histidine

Fusion tag:

C-His & AVI

Group:

recombinants

Expressed Host:

HEK293 Cells

Sequence:

Met 1-Met 210

Source:

Recombinants or rec. proteins

Purity:

＞95 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4

Endotoxin:

＜1.0 EU per µg as determined by the LAL method

Synonym:

CD32;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR2;FCGR2A1;IGFR2

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Activity:

Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM.3. Labeling ratio of biotin to protein: 0.1-0.6.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.