Recombinant Human MARK3 / CTAK1 / EMK-2 Protein (His & GST tag)

AP Mol Mass:

120 kDa

Conjugation:

histidine

Mol Mass:

109.3 kDa

Accession:

AAH24773.1

Fusion tag:

N-His & GST

Group:

recombinants

Sequence:

Met 1-Leu 729

Synonym:

CTAK1;KP78;Par-1a;PAR1A

Expressed Host:

Baculovirus-Insect Cells

Source:

Recombinants or rec. proteins

Purity:

＞88 % as determined by SDS-PAGE

Endotoxin:

＜1.0 EU per µg as determined by the LAL method

Formulation:

Supplied as sterile 20mM Tris, 500mM NaCl, 0.25mM DTT, 0.1mM PMSF, 0.1mM EDTA, pH 7.4, 10% gly

Activity:

The specific activity was determined to be 7 nmol/min/mg using synthetic CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

Shipping:

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

MAP / microtubule affinity-regulating kinase 3, also known as C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, MARK3, CTAK1 and EMK2, is a ubiquitous expressed protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and MARK subfamily. MARK3 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. The Par-1 / MARK protein kinases play a pivotal role in establishing cellular polarity. This family of kinases contains a unique domain architecture, in which a ubiquitin-associated (UBA) domain is located C-terminal to the kinase domain. MARKs / PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1).