Recombinant Human CD16b / FCGR3B Protein (His tag)

Activity:

NA

Fusion tag:

C-His

Mol Mass:

22.2 kDa

Conjugation:

histidine

AP Mol Mass:

50-55 kDa

Accession:

NP_000561.3

Group:

recombinants

Expressed Host:

HEK293 Cells

Sequence:

Met 1-Ser 200

Source:

Recombinants or rec. proteins

Purity:

> 95 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4

Synonym:

CD16;CD16b;Fc gamma RIIIb;FCG3;FCGR3;FCR-10;FCRIII;FCRIIIb

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.