Recombinant Human Low Affinity Fc Epsilon RII Protein

Activity:

NA

Fusion tag:

N-8His

Accession:

P06734

Mol Mass:

32.1 kDa

AP Mol Mass:

35-40 kDa

Expressed Host:

Human cells

Group:

recombinants

Sequence:

Asp48-Ser321

Source:

Recombinants or rec. proteins

Purity:

＞95% as determined by reducing SDS-PAGE.

Endotoxin:

＜1.0 EU per µg as determined by LAL test.

Formulation:

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Shipping:

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Test:

A high affinity purification column was use to purify Recombinant Fc Epsilon RII Protein by Elabscience by chromatographic size exclusion.

Synonym:

Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD23; FCER2; CD23A; CLEC4J; FCE2; IGEBF

Stability and Storage:

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

Low affinity immunoglobulin epsilon Fc receptor(CD23) is a secreted and single-pass type II membrane protein which is also exists as a soluble excreted form. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. Unlike many of the antibody receptors, CD23/FCER2 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. In flow cytometry, CD23/FCER2 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative). CD23/FCER2 can also be demonstrated in germinal centre B-cells using immunohistochemistry, but it is not present in the resting cells of the surrounding mantle zone. CD23/FCER2 has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specificantigen).