Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-B Protein

Fusion tag:

C-6His

AP Mol Mass:

27 kDa

Tissue:

region

Mol Mass:

20.6 kDa

Expressed Host:

Human cells

Sequence:

Thr43-Pro217

Group:

recombinants

Accession:

NP_001002274.1

Source:

Recombinants or rec. proteins

Purity:

＞95% as determined by reducing SDS-PAGE.

Endotoxin:

＜1.0 EU per µg as determined by LAL test.

Formulation:

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Shipping:

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Synonym:

Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b; IgG Fc Receptor II-b; CDw32; Fc-Gamma RII-b; Fc-Gamma-RIIb; FcRII-b; CD32; FCGR2B; FCG2; IGFR2

Test:

A high affinity purification column was use to purify Recombinant Immunoglobulin Gamma Fc Region Receptor II-B Protein by Elabscience by chromatographic size exclusion.

Activity:

Measured by its binding ability in a functional ELISA.Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b. The EC50 of Human CD32b is 1.6 - 3.7 μg/ml.2. Labeling ratio of biotin to protein: 0.5

Stability and Storage:

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description:

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Background:

FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.