Recombinant Human CD32b / FCGR2B Protein (His tag)

Fusion tag:

C-His

Mol Mass:

20.8 kDa

Conjugation:

histidine

AP Mol Mass:

25-30 kDa

Group:

recombinants

Expressed Host:

HEK293 Cells

Accession:

NP_001002274.1

Sequence:

Ala 46-Pro 217

Source:

Recombinants or rec. proteins

Synonym:

CD32;CD32B;Fc gamma RIIB;FCGR2

Purity:

> 95 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method

Activity:

Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6.4 μg/ml.

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G(2)/M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.