Recombinant Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor IV Protein

Fusion tag:

C-6His

Tissue:

region

Mol Mass:

21.9 kDa

AP Mol Mass:

28-35kDa

Accession:

NP_653142.2

Expressed Host:

Human Cells

Group:

recombinants

Latin name:

Mus musculus

Sequence:

Met 1-Gln 203

Source:

Recombinants or rec. proteins

Purity:

＞95% as determined by reducing SDS-PAGE.

Formulation:

Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.

Synonym:

Low Affinity Immunoglobulin Gamma Fc Region Receptor IV;FcgR4; CD16-2

Activity:

1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA.2. Labeling ratio of biotin to protein: 0.6

Stability and Storage:

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Test:

A high affinity purification column was use to purify Recombinant Mouse Immunoglobulin Gamma Fc Region Receptor IV Protein by Elabscience by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Description:

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Background:

Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc γ receptors (FcR), and they are Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc γ RIII (CD16) group.