Recombinant Mouse CD32 / FCGR2B Protein (His tag) 100µg

Fusion tag:

C-His

Mol Mass:

21.7 kDa

Conjugation:

histidine

AP Mol Mass:

35-40 kDa

Group:

recombinants

Latin name:

Mus musculus

Expressed Host:

HEK293 Cells

Sequence:

Met 1-Arg 217

Accession:

NP_001070657.1

Source:

Recombinants or rec. proteins

Purity:

> 98 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method

Synonym:

AI528646;CD32;F630109E10Rik;Fcgr2;Fcgr2a;FcgRII;Fcr-2;Fcr-3;fcRII;Fc[g]RII;Ly-17;Ly-m20;LyM-1

Activity:

Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR2B-His (CD32) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml.

Stability and Storage:

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping:

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Test:

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background:

Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.