100µg
PKSR030402-100µg
716 EUR
Fusion tag:
C-His
Accession:
Q63203-1
Mol Mass:
21.9 kDa
Conjugation:
histidine
AP Mol Mass:
35-40 KDa
Synonym:
FCRII;Fcgr2
ExpressedHost:
HEK293 Cells
Group:
recombinants
Sequence:
Met 1-Pro 212
Latin name:
Rattus norvegicus
Source:
Recombinants or rec. proteins
Purity:
> 97 % as determined by SDS-PAGE
Formulation:
Lyophilized from sterile PBS, pH 7.4
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human rat FCGR2B-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.3-0.8 μg/ml.
Stability and Storage:
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
About:
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Background:
FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.